Miike T, Sakamoto Y, Sakamoto Y, Matsuoka A, Shinada K, Nakayama K, Sakurai R, Asahi M, Yoshitake K, Narumi S, Koba M, Nagashima F, Inoue S, et al.
Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, Inc. Date of publication 2020 Oct 1;volume 47(4):591-595.
1. Undersea Hyperb Med. 2020 Fourth Quarter;47(4):591-595. doi:
10.22462/10.12.2020.8.
Influence of hyperbaric oxygen therapy on thrombus formation ability in humans.
Miike T(1), Sakamoto Y(1), Sakamoto Y(1), Matsuoka A(1), Shinada K(1), Nakayama
K(1), Sakurai R(1), Asahi M(1), Yoshitake K(1), Narumi S(1), Koba M(1),
Nagashima F(1), Inoue S(1).
Author information:
(1)Department of Emergency and Critical Care Medicine, Faculty of Medicine, Saga
University, Saga City, Saga Prefecture, Japan.
BACKGROUND: Hyperbaric oxygen (HBO2) therapy was introduced nearly 300 years
ago. However, its effect on thrombus formation is unclear. This may be because
platelet and coagulation functions are unstable, yielding variable results;
hence, accurate measurement is difficult. Our study aimed to analyze changes in
thrombus formation before and after HBO2 therapy by using a total thrombus
formation analysis system (TTAS).
METHODS: Six patients were prescribed HBO2 therapy for skin and soft tissue
ulcers, and necrotic fasciitis. Blood samples were collected immediately before
and after treatment. Then samples were put into a reservoir that connected to
AR-chip to assess changes in the thrombus formation ability of both platelets
and coagulation factors. We examined the differences in the thrombus formation
ability using T-TAS. Time until the onset of white thrombus formation (T10) and
complete occlusion of the capillary (T80) were analyzed by a two-way repeated
measure analysis of variance (ANOVA).
RESULTS: The duration to pressure increase of samples after HBO2 therapy was
longer than the duration before HBO2 therapy (p<0.05). This suggests decreased
clot adhesiveness to the inner surface of the simulated blood vessel and reduced
clot formation ability.
CONCLUSIONS: The results for T10 and T80 suggest that HBO2 therapy reduced
thrombus formation ability in the enrolled patients. We believe that T-TAS is a
promising method to predict the efficacy of HBO2 therapy.
Copyright© Undersea and Hyperbaric Medical Society.
DOI: 10.22462/10.12.2020.8
PMID: 33227835 [Indexed for MEDLINE]
Conflict of interest statement: The authors of this paper declare no conflicts
of interest exist with this submission.
