Dasgupta A, Orgill D, Galiano RD, Zelen CM, Huang YC, Chnari E, Li WW, et al.
Plastic and reconstructive surgery. Global open. Date of publication 2016 Oct 4;volume 4(10):e1065.
1. Plast Reconstr Surg Glob Open. 2016 Oct 4;4(10):e1065. eCollection 2016 Oct.
A Novel Reticular Dermal Graft Leverages Architectural and Biological Properties
to Support Wound Repair.
Dasgupta A(1), Orgill D(1), Galiano RD(1), Zelen CM(1), Huang YC(1), Chnari E(1),
Li WW(1).
Author information:
(1)Musculoskeletal Transplant Foundation, Edison, N.J.; Department of Surgery,
Brigham and Women's Hospital, Boston, Mass.; Department of Surgery, Harvard
Medical School, Boston, Mass.; Division of Plastic Surgery, Northwestern
University Feinberg School of Medicine, Chicago, Ill.; Professional Education and
Research Institute, Roanoke, Va.; and The Angiogenesis Foundation, Cambridge,
Mass.
BACKGROUND: Acellular dermal matrices (ADMs) are frequently used in
reconstructive surgery and as scaffolds to treat chronic wounds. The
3-dimensional architecture and extracellular matrix provide structural and
signaling cues for repair and remodeling. However, most ADMs are not uniformly
porous, which can lead to heterogeneous host engraftment. In this study, we
hypothesized that a novel human reticular ADM (HR-ADM; AlloPatch Pliable,
Musculoskeletal Transplant Foundation, Edison, N.J.) when aseptically processed
would have a more open uniform structure with retention of biological components
known to facilitate wound healing.
METHODS: The reticular and papillary layers were compared through histology and
scanning electron microscopy. Biomechanical properties were assessed through
tensile testing. The impact of aseptic processing was evaluated by comparing
unprocessed with processed reticular grafts. In vitro cell culture on fibroblasts
and endothelial cells were performed to showcase functional cell activities on
HR-ADMs.
RESULTS: Aseptically processed HR-ADMs have an open, interconnected uniform
scaffold with preserved collagens, elastin, glycosaminoglycans, and hyaluronic
acid. HR-ADMs had significantly lower ultimate tensile strength and Young's
modulus versus the papillary layer, with a higher percentage elongation at break,
providing graft flexibility. These preserved biological components facilitated
fibroblast and endothelial cell attachment, cell infiltration, and new matrix
synthesis (collagen IV, fibronectin, von Willebrand factor), which support
granulation and angiogenic activities.
CONCLUSIONS: The novel HR-ADMs provide an open, interconnected scaffold with
native dermal mechanical and biological properties. Furthermore, aseptic
processing retains key extracellular matrix elements in an organized framework
and supports functional activities of fibroblasts and endothelial cells.
DOI: 10.1097/GOX.0000000000001065
PMCID: PMC5096524
PMID: 27826469
