Tattini C, Manchio J, Zaporojan V, Carderelli G, Bonassar L, Spangenberger A, Weinzweig J, et al.
Plastic and reconstructive surgery. Date of publication 2008 Oct 1;volume 122(4):1036-45.
1. Plast Reconstr Surg. 2008 Oct;122(4):1036-45. doi: 10.1097/PRS.0b013e3181858bf0.
Role of TGF-beta and FGF in the treatment of radiation-impaired wounds using a
novel drug delivery system.
Tattini C(1), Manchio J, Zaporojan V, Carderelli G, Bonassar L, Spangenberger A,
Weinzweig J.
Author information:
(1)Department of Plastic and Reconstructive Surgery, Craniofacial Biology and
Tissue Engineering Laboratory, Lahey Clinic Medical Center, Burlington,
Massachussetts 01805, , USA.
BACKGROUND: Despite refinements in radiotherapy, radiation-impaired wound healing
continues to be a major source of postoperative morbidity with few treatment
options. The application of polypeptide growth factors has been investigated in
both the clinical and laboratory settings. The authors used a novel
sustained-release delivery system to examine the effect of transforming growth
factor (TGF)-beta and fibroblast growth factor (FGF) on radiation-impaired wound
healing in a rodent model.
METHODS: Eighty Sprague-Dawley rats underwent dorsal skin surface irradiation of
2500 cGy using a medical linear accelerator producing energy of 6 MeV followed by
creation of a full-thickness skin incision. Six groups of 16 animals underwent
either sham irradiation (irradiation control); irradiation only; irradiation and
unimpregnated delivery system only; or irradiation and either TGF-beta, FGF, or
TGF-beta plus FGF combined. Four animals from each group were euthanized at 4, 7,
14, and 28 days, and the harvested specimens underwent ultimate tensile strength
testing and histologic evaluation.
RESULTS: All five irradiated groups had significantly lower ultimate tensile
strength than the sham-irradiated control group at all time points (p < 0.05),
thus validating the authors' model of radiation-impaired wound healing.
Functional analysis demonstrated that all three growth factor-treated groups had
significantly higher tensile strengths than either of the untreated irradiated
groups at 14 days after wounding (p < 0.05). Histologic evaluation of the
irradiated groups revealed increased cellularity and more organized collagen
architecture of all treated groups when compared with the untreated groups, with
the most pronounced differences seen at 7 days and 14 days after wounding.
CONCLUSIONS: This study effectively demonstrates that TGF-beta and FGF act
individually and synergistically when delivered locally by means of a sustained
release system to improve ultimate tensile strength in an acute postirradiation
impaired wound-healing model.
DOI: 10.1097/PRS.0b013e3181858bf0
PMID: 18827634 [Indexed for MEDLINE]
